The appeal was lodged in court just hours before a deadline which would have forced the company to stop operating in the capital immediately.
Uber could continue to be hailed across London for another year after officially filing its appeal against Transport for London's decision to revoke its London license.
Uber CEO Dara Khosrowshahi has held talks with London's transport agency in a bid to keep the service operational.
Today was the last day Uber could appeal TfL's decision to renew its licence and now in the hands of the courts, Londoners could see Uber stick around in the future. "As our new CEO [chief executive] has said, we are determined to make things right", an Uber spokesperson said.
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TfL has a number of concerns about Uber, including its approach to reporting serious criminal offences, how drivers' medical certificates are obtained, how criminal record checks are carried out, and its use of technology which allegedly helps it to evade law enforcement officials.
Previous to this, Khosrowshahi penned an open letter to London in the Evening Standard apologising for the mistakes that lead to Uber having its licence yanked. The pair are said to have met on 3 October, and both sides have since described the talks as "constructive".
His predecessor, Travis Kalanick, left the company following allegations that managers didn't adequately address reports of sexual harassment.
"While Uber had revolutionised the way people move in cities around the world, it's equally true that we've got things wrong along the way", he wrote.
Khan, a politician from the opposition Labour Party who is also chairman of TfL, has long criticised Uber's British management and backed the regulator's decision on Thursday.